pp2 (a selective inhibitor of src family kinases)  (Enzo Biochem)

Journal: Molecular Medicine

Article Title: CD36 deficiency ameliorates drug-induced acute liver injury in mice

doi: 10.1186/s10020-021-00325-z

Figure Lengend Snippet: Treatment with PP2 attenuated APAP induced liver injury. A Male C57 mice were starved for 16 h and i.p. injected with/without PP2 (1.5 mg/kg) prior to APAP administration (300 mg/kg). Serum was collected after 8 h and 24 h for ALT and AST measurement. B H&E staining in liver section from the mice at 24 h after APAP injection. *P < 0.05, **P < 0.01, ***P < 0.001. N = 6 mice per group

Article Snippet: The Src family kinase selective inhibitor PP2 (Selleck, China) was injected to the mice intraperitoneally (1.5 mg/kg, dissolved in ethanol) at half an hour prior to APAP-treatment.

Techniques: Injection, Staining

Journal: PLoS ONE

Article Title: Low-Density Lipoprotein Receptor Related protein-1 (LRP1)-Dependent Cell Signaling Promotes Neurotrophic Activity in Embryonic Sensory Neurons

doi: 10.1371/journal.pone.0075497

Figure Lengend Snippet: A , Phase-contrast images of cultured sensory neurons after 2 weeks of treatment. Note the reduction of sprouting in RBD (100 nM) treated cultures, but not NGF (50 ng/ml) treated cultures with treatment of PP2 (1 µM) for 30 min prior to the addition of factors. Scale bar, 50 µm. B , Levels of GAP-43 mRNA in primary cultures treated with RBD (100 nM) or NGF (50 ng/ml) for 2 weeks, with or without PP2 treatment. * p <0.05 (n=3-4 independent experiments).

Article Snippet: The Src-family kinase selective inhibitor (SFK), (PP2); 3-(4-chlorophenyl) 1-(1,1-dimethyl)-H-pyrazolo[3,-d] pyrimidine-4-amine), was purchased from Calbiochem (San Diego, CA).

Techniques: Cell Culture